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Chromosome 5 sequence imputation and accuracy assessmentHaving estimated the location of the chromosome 5 QTL, we then imputed sequence based genotype data into a 10Mbp interval centred on the BovineHD0500026662 SNP for a population of 64,244 animals. This population comprised the 42,933 animals used for the original GWAS, and an additional 21,311 animals (of similar breed composition) typed predominantly using the Super GGP BeadChip. The sequence reference panel consisted of 556 animals at an average of 15 genome wide mapped read depth.

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